| TH

Tyrosine hydroxylase (TH), also known as tyrosine 3-monooxygenase, is the enzyme responsible for catalyzing the conversion of the amino acid L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA). It utilizes molecular oxygen (O2) and cofactors such as iron (Fe2+) and tetrahydrobiopterin. L-DOPA is a precursor to dopamine, which in turn is a precursor to the important neurotransmitters norepinephrine and epinephrine. This enzyme catalyzes the rate-limiting step in the synthesis of these catecholamines. The enzyme is encoded by the TH gene and is found in the central nervous system (CNS), peripheral sympathetic neurons, and adrenal medulla.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.36	1.78	1.23	0.79	0.46	0.29	0.17	0.09
Corrected OD	2.27	1.69	1.14	0.7	0.37	0.2	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human TH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	83-113	94
Cell Lysates(n=8)	88-105	98

 

| Linearity

Samples were spiked with high concentrations of Human TH and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	86-104	99-108
	Average (%)	97	104
1 : 4	Range (%)	96-114	84-112
	Average (%)	103	103