| PZP

Pregnancy zone protein (PZP), also known as the pregnancy-associated α2-glycoprotein (α2-PAG or PAα2G),is a protein which is encoded by the PZP gene on chromosome 12.PZP is part of the alpha-2 globulin family of proteins.It is often associated with pregnancy, during which it can be the most abundant among the plasma proteins.Pregnancy zone proteins (PZPs) exist in the functionally active form as homodimers of 360 kDa molecular weights. This dimerisation of the constituent 180 kDa monomers happens by means of disulfide bridge bonds. PZPs are secreted in highly glycosylated form.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.48	1.65	1.07	0.71	0.45	0.28	0.19	0.09
Corrected OD	2.39	1.56	0.98	0.62	0.36	0.19	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human PZP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human PZP and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-98	90-110
	Average (%)	91	95	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108