Home > Sandwich ELISA Kits > Human Mucosae Associated Epithelia Chemokine (MEC;CCL28) ELISA Kit

Product Name:

Human Mucosae Associated Epithelia Chemokine (MEC;CCL28) ELISA Kit

Cat.#:

JL11426

Regulatory Status:

RUO

Alternative:

CCL28; SCYA28; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 28; Chemokine C-C-Motif Ligand 28; Small-inducible cytokine A28

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.05ng/mL

Specificity:

This kit recognizes Human MEC;CCL28 in samples. No significant cross-reactivity or interference between Human MEC;CCL28 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MEC;CCL28

Chemokine (C-C motif) ligand 28 (CCL28), also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.35	1.35	0.8	0.48	0.34	0.26	0.22	0.09
Corrected OD	2.26	1.26	0.71	0.39	0.25	0.17	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MEC;CCL28 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human MEC;CCL28 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-97	90-110
1 : 2	Average (%)	91	94	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108