Human Chemokine C-C-Motif Ligand 1 (CCL1) ELISA Kit

Cat.#:

JL15076

Regulatory Status:

RUO

Alternative:

SCYA1; I-309; TCA3; P500; SISe; Small Inducible Cytokine A1; T Lymphocyte Secreted Protein I-309

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

28.45pg/mL

Specificity:

This kit recognizes Human CCL1 in samples. No significant cross-reactivity or interference between Human CCL1 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| CCL1

Chemokine (C-C motif) ligand 1 (CCL1) is also known as small inducible cytokine A1 and I-309 in humans. CCL1 is a small glycoprotein that belongs to the CC chemokine family.CCL1 is a small glycoprotein with a molecular weight of approximately 15-16 kDa.CCL1 is secreted by activated monocytes/macrophages, T lymphocytes and endothelial cells.CCL1 binds to the chemokine receptor CCR8 and induces Ca2+ influx, chemotaxis and regulate apoptosis.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	2.56	1.77	1.22	0.71	0.46	0.31	0.23	0.07
Corrected OD	2.49	1.7	1.15	0.64	0.39	0.24	0.16	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CCL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-107	104

| Linearity

Samples were spiked with high concentrations of Human CCL1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	89-96	90-110
1 : 2	Average (%)	91	94	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108