Home > Sandwich ELISA Kits > Human Monocyte Chemotactic Protein 2 (MCP-2;CCL8) ELISA Kit

Product Name:

Human Monocyte Chemotactic Protein 2 (MCP-2;CCL8) ELISA Kit

Cat.#:

JL13264

Regulatory Status:

RUO

Alternative:

CCL8; CCL-8; SCYA8; MCP-2; HC14; Chemokine C-C-Motif Ligand 8; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 8; Monocyte Chemoattractant Protein 2

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

15.7pg/mL

Specificity:

This kit recognizes Human MCP-2;CCL8 in samples. No significant cross-reactivity or interference between Human MCP-2;CCL8 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MCP-2;CCL8

Chemokine (C-C motif) ligand 8 (CCL8), also known as monocyte chemoattractant protein 2 (MCP2), is a protein that is encoded by the CCL8 gene.CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.36	1.36	0.8	0.48	0.34	0.28	0.23	0.09
Corrected OD	2.27	1.27	0.71	0.39	0.25	0.19	0.14	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MCP-2;CCL8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human MCP-2;CCL8 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-94	90-110
1 : 2	Average (%)	91	91	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108