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Human Immunosuppressive GlycoProteins (Glycodelin) ELISA Kit

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Product Name:

Human Immunosuppressive GlycoProteins (Glycodelin) ELISA Kit

Cat.#:

JL19679

Regulatory Status:
Alternative:

Glycodelin;PAEP;GD; GdA; GdF; GdS; PAEG; PEP; PP14; Glycodelin; Placental Protein 14; Alpha Uterine Protein; Pregnancy-Associated Endometrial Alpha-2-Globulin; Progesterone-associated

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
4.68-300ng/mL
Sensitivity:
2.4ng/mL
Specificity:
This kit recognizes Human Glycodelin in samples. No significant cross-reactivity or interference between Human Glycodelin and analogues was observed.
Sample Type:
Serum;plasma;tissue homogenates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Immunology
  • Human Immunosuppressive GlycoProteins (Glycodelin) ELISA Kit
  • Human Immunosuppressive GlycoProteins (Glycodelin) ELISA Kit
  • Human Immunosuppressive GlycoProteins (Glycodelin) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| Glycodelin

Glycodelin (GD) also known as human placental protein-14 (PP-14) progestogen-associated endometrial protein (PAEP) or pregnancy-associated endometrial alpha-2 globulin is a glycoprotein that inhibits cell immune function and plays an essential role in the pregnancy process. In humans is encoded by the PAEP gene.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 300 150 75 37.5 18.75 9.37 4.68 0
OD 2.56 1.45 0.8 0.48 0.33 0.23 0.2 0.08
Corrected OD 2.48 1.37 0.72 0.4 0.25 0.15 0.12 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human Glycodelin spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Serum(n=8) 84-101 96
Plasma(n=8) 92-105 102
Tissue Homogenates(n=8) 96-108 105





 

| Linearity

Samples were spiked with high concentrations of Human Glycodelin and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Serum(n=4) Plasma(n=4) Tissue Homogenates(n=4)
1 : 2 Range (%) 84-95 88-96 90-110
Average (%) 91 93 96
1 : 4 Range (%) 89-103 87-108 105-115
Average (%) 94 98 108







 
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Sample Data
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Sample Data
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Sample Data
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460 / 460
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