Human Immunoglobulin A (IgA) ELISA Kit

Cat.#:

JL12695

Regulatory Status:

RUO

Alternative:

Immunoglobulin A

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.39-25ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human IgA in samples. No significant cross-reactivity or interference between Human IgA and analogues was observed.

Sample Type:

Serum;plasma;saliva and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| IgA

Immunoglobulin A (Ig A, also referred to as sIgA in its secretory form) is an antibody that plays a role in the immune function of mucous membranes. The amount of IgA produced in association with mucosal membranes is greater than all other types of antibody combined. In absolute terms, between three and five grams are secreted into the intestinal lumen each day. This represents up to 15% of total immunoglobulins produced throughout the body.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human IgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Saliva(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human IgA and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Saliva(n=4)
1 : 2	Range (%)	84-95	88-96	89-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108