Home > Sandwich ELISA Kits > Human Nitric Oxide Synthase 3, Endothelial (NOS3) ELISA Kit

Product Name:

Human Nitric Oxide Synthase 3, Endothelial (NOS3) ELISA Kit

Cat.#:

JL14999

Regulatory Status:

RUO

Alternative:

NOS3; ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS; Nitric Oxide Synthase 3; Endothelial

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

62.5-4000pg/mL

Sensitivity:

31.8pg/mL

Specificity:

This kit recognizes Human NOS3 in samples. No significant cross-reactivity or interference between Human NOS3 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| NOS3

Endothelial NOS (eNOS) also know as nitric Oxide Synthase 3, Endothelial (NOS3) or constitutive NOS (cNOS), is an enzyme that is encoded by the NOS3 gene located in the 7q35-7q36 region of chromosome 7.This enzyme is one of three isoforms that synthesize nitric oxide (NO), a small gaseous and lipophilic molecule that participates in several biological processes.eNOS is primarily responsible for the generation of NO in the vascular endothelium, a monolayer of flat cells lining the interior surface of blood vessels, at the interface between circulating blood in the lumen and the remainder of the vessel wall.NO produced by eNOS in the vascular endothelium plays crucial roles in regulating vascular tone, cellular proliferation, leukocyte adhesion, and platelet aggregation.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	4000	2000	1000	500	250	125	62.5	0
OD	2.65	1.92	1.38	0.95	0.61	0.39	0.23	0.08
Corrected OD	2.57	1.84	1.3	0.87	0.53	0.31	0.15	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human NOS3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human NOS3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	89-110
1 : 2	Average (%)	91	92	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108