Product Name:
Human Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit
Cat.#:
JL11296
Regulatory Status:
Alternative:
MDGI; FABP11; H-FABP; O-FABP; Hfabp; FABP3; Fatty Acid Binding Protein 3, Muscle And Heart; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-ty
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
7.8-500pg/mL
Sensitivity:
3.6pg/mL
Specificity:
This kit recognizes Human FABP3 in samples. No significant cross-reactivity or interference between Human FABP3 and analogues was observed.
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure:

Research Area:
Metabolism
Product Datasheets
| FABP3
Heart-type fatty acid binding protein (H-FABP), also known as mammary growth inhibitor, is a protein encoded by the FABP3 gene. Heart-type fatty acid binding protein (H-FABP) is a small cytoplasmic protein (15 kDa) released by cardiomyocytes after ischemia.Like the other 9 different FABPs identified, H-FABP is involved in active fatty acid metabolism, where it transports fatty acids from the cell membrane to the mitochondria for oxidation.FABP3, in interacting with cardiac troponin I, interacts with TNNI3K Interacts with TNNI3K, the protein also interacts with VPS28, KIAA159, NUP62, PLK1, UBC, and Xpo1.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (pg/mL) | 500 | 250 | 125 | 62.5 | 31.2 | 15.6 | 7.8 | 0 |
OD | 2.28 | 1.63 | 1.04 | 0.69 | 0.45 | 0.3 | 0.22 | 0.09 |
Corrected OD | 2.19 | 1.54 | 0.95 | 0.6 | 0.36 | 0.21 | 0.13 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Human FABP3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Serum(n=8) | 84-101 | 96 |
Plasma(n=8) | 92-105 | 102 |
Cell Culture Supernates(n=8) | 97-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Human FABP3 and diluted with Universal Diluent to produce samples with values within the range of the assay.Serum(n=4) | Plasma(n=4) | Cell Culture Supernates(n=4) | ||
1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
Average (%) | 91 | 93 | 96 | |
1 : 4 | Range (%) | 89-103 | 87-108 | 105-115 |
Average (%) | 95 | 98 | 108 |






Size
Quantity
Price ($)
48T


360 / 360
96T


460 / 460