Human Prostaglandin Reductase 2 (PTGR2) ELISA Kit

Cat.#:

JL16917

Regulatory Status:

RUO

Alternative:

ZADH1; Zinc Binding Alcohol Dehydrogenase Domain Containing 1; 15-oxoprostaglandin 13-reductase; Zinc-binding alcohol dehydrogenase domain-containing protein 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.071ng/mL

Specificity:

This kit recognizes Human PTGR2 in samples. No significant cross-reactivity or interference between Human PTGR2 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PTGR2

Prostaglandin Reductase 2(PTGR2) is an enzyme involved in the metabolism of prostaglandins.Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation (By similarity).Diseases associated with PTGR2 include Primary Hypertrophic Osteoarthropathy.An important paralog of this gene is PTGR1.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.44	1.91	1.37	0.84	0.51	0.34	0.26	0.07
Corrected OD	2.37	1.84	1.3	0.77	0.44	0.27	0.19	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human PTGR2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	95
Cell Culture Supernates(n=8)	94-105	101

| Linearity

Samples were spiked with high concentrations of Human PTGR2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-98
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	96	98