| sFASL

Factor Related Apoptosis Ligand (FASLor CD95L) is a type II transmembrane protein belonging to the tumor necrosis factor superfamily (TNFSF) It binds with its receptor, called FAS receptor (also called CD95) and plays a crucial role in the regulation of the immune system and in induction of apoptosis, a programmed cell death.FasL is expressed on various cell types, including T cells, natural killer cells, monocytes, neutrophils, and vascular endothelial cells.Its functions include: cytotoxic T cell activity, immune privilege, maternal tolerance, and tumor counterattack.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	500	250	125	62.5	31.2	15.6	7.81	0
OD	2.23	1.42	0.83	0.5	0.31	0.23	0.18	0.08
Corrected OD	2.15	1.34	0.75	0.42	0.23	0.15	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human sFASL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	95
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human sFASL and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-95	90-111
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	86-108	105-114
	Average (%)	94	98	107