| FUNDC1

FUN14 domain containing 1 is a protein that is encoded by the FUNDC1 gene. FUN14 domain containing 1 (FUNDC1) is a mitophagy receptor. A pivotal role for FUNDC1 in the maintenance of hepatic homeostasis. FUNDC1-dependent mitophagy suppressed hepatocarcinogenesis through amelioration of inflammasome activation. In addition, downregulation of FUNDC1 suppressed mitophagy in alcoholic liver diseases, which activated the DNA-dependent protein kinase catalytic subunit (DNA-PKcs)-p53-dynamin-related protein 1 (Drp1) signaling cascade, leading to unchecked mitochondrial fission and liver injury.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.01
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human FUNDC1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human FUNDC1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	90-110
	Average (%)	91	96
1 : 4	Range (%)	89-103	105-114
	Average (%)	94	108