Product Name:
Human YTH Domain Family Protein 3 (YTHDF3) ELISA Kit
Cat.#:
JL52826
Regulatory Status:
Alternative:
YTH N6-methyladenosine RNA binding protein 3; DF3
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.074ng/mL
Specificity:
This kit recognizes Human YTHDF3 in samples. No significant cross-reactivity or interference between Human YTHDF3 and analogues was observed.
Sample Type:
Tissue homogenates;cell lysates and other biological fluids
Assay Procedure:
Research Area:
Cell Biology
Product Datasheets
| YTHDF3
YTH domain family protein 3 is a protein that is encoded by the YTHDF3 gene. This gene encodes a member of the YTH (YT521-B homology) domain protein family. The YTH domain is common in eukaryotes, is often found in the middle of the protein sequence, and may function in binding to RNA. Alternative splicing results in multiple transcript variants.
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Human YTHDF3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Sample Type | Range(%) | Average Recovery(%) |
| Tissue Homogenates(n=8) | 84-101 | 94 |
| Cell Lysates(n=8) | 96-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Human YTHDF3 and diluted with Universal Diluent to produce samples with values within the range of the assay.| Tissue Homogenates(n=4) | Cell Lysates(n=4) | ||
| 1 : 2 | Range (%) | 84-95 | 90-110 |
| Average (%) | 91 | 96 | |
| 1 : 4 | Range (%) | 89-103 | 105-114 |
| Average (%) | 94 | 107 |
Size
Quantity
Price ($)
48T
1
360 / 360
96T
1
460 / 460


