Human Collagen Type III Alpha 1 (COL3α1) ELISA Kit

Cat.#:

JL33934

Regulatory Status:

RUO

Alternative:

COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1(III)chain

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

6.25-400ng/mL

Sensitivity:

2.35ng/mL

Specificity:

This kit recognizes Human COL3α1 in samples. No significant cross-reactivity or interference between Human COL3α1 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| COL3α1

Type III Collagen is a homotrimer, or a protein composed of three identical peptide chains (monomers), each called an alpha 1 chain of type III collagen. Formally, the monomers are called collagen type III, alpha-1 chain and in humans are encoded by the COL3A1 gene. Type III collagen is one of the fibrillar collagens whose proteins have a long, inflexible, triple-helical domain.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	400	200	100	50	25	12.5	6.25	0
OD	2.3	1.87	1.25	0.82	0.55	0.41	0.32	0.09
Corrected OD	2.21	1.78	1.16	0.73	0.46	0.32	0.23	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human COL3α1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human COL3α1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108