Human Pleiotrophin (PTN) ELISA Kit

Cat.#:

JL10184

Regulatory Status:

RUO

Alternative:

HARP; HBGF8; HBNF; NEGF1; Heparin Binding Growth Factor 8; Neurite Growth-Promoting Factor 1; Heparin Affin Regulatory Peptide; Heparin Binding Growth Associated Molecule

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.2-2000pg/mL

Sensitivity:

16.2pg/mL

Specificity:

This kit recognizes Human PTN in samples. No significant cross-reactivity or interference between Human PTN and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PTN

Pleiotrophin (PTN) also known as heparin-binding brain mitogen (HBBM) or heparin-binding growth factor 8 (HBGF-8) or neurite growth-promoting factor 1 (NEGF1) or heparin affinity regulatory peptide (HARP) or heparin binding growth associated molecule (HB-GAM) is a protein that is encoded by the PTN gene. Pleiotrophin is an 18-kDa growth factor that has a high affinity for heparin. It is structurally related to midkine and retinoic acid induced heparin-binding protein.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human PTN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	85-101	96
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human PTN and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108