Home > Sandwich ELISA Kits > Human Interferon Gamma Inducible Protein 30 (IFI30) ELISA Kit

Product Name:

Human Interferon Gamma Inducible Protein 30 (IFI30) ELISA Kit

Cat.#:

JL13661

Regulatory Status:

RUO

Alternative:

GILT; IP30; Legumaturain; Gamma-Interferon-Inducible Lysosomal Thiol Reductase; Gamma-interferon-inducible lysosomal thiol reductase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.1ng/mL

Specificity:

This kit recognizes Human IFI30 in samples. No significant cross-reactivity or interference between Human IFI30 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| IFI30

Gamma-interferon-inducible lysosomal thiol reductase is an enzyme that is encoded by the IFI30 gene.The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.36	1.79	1.28	0.86	0.63	0.38	0.26	0.08
Corrected OD	2.28	1.71	1.2	0.78	0.55	0.3	0.18	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human IFI30 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	93-105	102
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human IFI30 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-96	90-110
1 : 2	Average (%)	92	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	108