Home > Sandwich ELISA Kits > Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) ELISA Kit

Product Name:

Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) ELISA Kit

Cat.#:

JL30900

Regulatory Status:

RUO

Alternative:

Wnt7a; Wnt-7a; Protein Wnt-7a

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.2-2000pg/mL

Sensitivity:

15.2pg/mL

Specificity:

This kit recognizes Human WNT7A in samples. No significant cross-reactivity or interference between Human WNT7A and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Stem Cells

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| WNT7A

Protein Wnt-7a is a protein that is encoded by the WNT7A gene.The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family.This gene not only guides the development of the anterior-posterior axis in the female reproductive tract but also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. It is also responsive to changes in the levels of sex steroid hormone in the female reproductive tract. Decreased expression of this gene in human uterine leiomyoma is found to be inversely associated with the expression of estrogen receptor alpha.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.2	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human WNT7A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	85-101	96
Cell Lysates(n=8)	92-105	103

| Linearity

Samples were spiked with high concentrations of Human WNT7A and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98