| UQCR

UQCR11 (ubiquinol-cytochrome c reductase, complex III sub-unit XI) is a protein that in humans is encoded by the UQCR11 gene.UQCR11 is the smallest known component of Complex III in the mitochondrial respiratory chain.This gene encodes the smallest known component of the ubiquinol-cytochrome c reductase complex, which is also known as Complex III and is part of the mitochondrial respiratory chain.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.125	1.562	0.781	0
OD	2.58	1.86	1.14	0.72	0.46	0.3	0.22	0.09
Corrected OD	2.49	1.77	1.05	0.63	0.37	0.21	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human UQCR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	81-101	96
Cell Lysates(n=8)	92-105	100

 

| Linearity

Samples were spiked with high concentrations of Human UQCR and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	85-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-109
	Average (%)	94	102