| GLYT1

Sodium- and chloride-dependent glycine transporter 1, also known as glycine transporter 1, is a protein that is encoded by the SLC6A9 gene which is promising therapeutic target for treatment of diabetes and obesity. Elevation of extracellular synaptic glycine concentration by blockade of GlyT1 has been hypothesized to potentiate NMDA receptor function in vivo and to represent a rational approach for the treatment of schizophrenia and cognitive disorders. Several drug candidates have reached clinical trials.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.2	1.65	1.16	0.8	0.54	0.37	0.28	0.09
Corrected OD	2.11	1.56	1.07	0.71	0.45	0.28	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human GLYT1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	90-105	103

 

| Linearity

Samples were spiked with high concentrations of Human GLYT1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	88-98
	Average (%)	93
1 : 4	Range (%)	87-108
	Average (%)	99