Human Leptin Receptor (LEPR) ELISA Kit

Cat.#:

JL15154

Regulatory Status:

RUO

Alternative:

CD295; LEPR; OBR; LR

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

29.35pg/mL

Specificity:

This kit recognizes Human LEPR in samples. No significant cross-reactivity or interference between Human LEPR and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| LEPR

Leptin receptor, also known as LEP-R or OB-R, is a type I cytokine receptor,a protein that is encoded by the LEPR gene. LEP-R functions as a receptor for the fat cell-specific hormone leptin. LEP-R has also been designated as CD295 (cluster of differentiation 295). Its location is the cell membrane, and it has extracellular, trans-membrane and intracellular sections (protein regions).

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human LEPR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human LEPR and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108