Human Uncarboxylated Osteocalcin (ucOC) ELISA Kit

Cat.#:

JL14112

Regulatory Status:

RUO

Alternative:

ucOC

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.78-50ng/mL

Sensitivity:

0.41ng/mL

Specificity:

This kit recognizes Human ucOC in samples. No significant cross-reactivity or interference between Human ucOC and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| ucOC

Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone found in bone and dentin, first identified as a calcium-binding protein.Osteocalcin is secreted solely by osteoblasts and is thought to play a role in the body's metabolic regulation.[12] In its carboxylated form, calcium is bound directly to the bone and thus concentrates here.Because osteocalcin has gla domains, its synthesis is vitamin K2-dependent.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human ucOC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human ucOC and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108