Home > Sandwich ELISA Kits > Human Transcription Activator BRG1 (SMARCA4) ELISA Kit

Product Name:

Human Transcription Activator BRG1 (SMARCA4) ELISA Kit

Cat.#:

JL22217

Regulatory Status:

RUO

Alternative:

ATP dependent helicase SMARCA4;ATP-dependent helicase SMARCA4;BAF 190;BAF190;BAF190A;Brahma protein like 1;BRG1;BRG1 associated factor 190A;BRG1 protein;BRG1-associated factor 190A;BRM/SWI2 related gene 1;Global transcription activator homologous sequence

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.077ng/mL

Specificity:

This kit recognizes Human SMARCA4 in samples. No significant cross-reactivity or interference between Human SMARCA4 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SMARCA4

Transcription activator BRG1 also known as ATP-dependent chromatin remodeler SMARCA4 is a protein that is encoded by the SMARCA4 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SMARCA4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	95
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human SMARCA4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	91-110
1 : 2	Average (%)	91	96
1 : 4	Range (%)	89-103	105-114
1 : 4	Average (%)	94	107