| SMPD1

Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that is encoded by the SMPD1 gene.Sphingomyelin phosphodiesterase 1 belongs to the sphingomyelin phosphodiesterase family.Defects in the SMPD1 gene cause Niemann–Pick disease, SMPD1-associated.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.26	1.45	0.85	0.55	0.36	0.27	0.19	0.07
Corrected OD	2.19	1.38	0.78	0.48	0.29	0.2	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human SMPD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-107	104
Tissue Homogenates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human SMPD1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-97	88-96	90-110
	Average (%)	93	93	95
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108