Human Interleukin 9 (IL-9) ELISA Kit

Cat.#:

JL19292

Regulatory Status:

RUO

Alternative:

HP40; P40; TCGFIII; TCGF3; T Cell Growth Factor III; P40 T-Cell And Mast Cell Growth Factor; P40 Cytokine; Homolog Of Mouse T Cell And Mast Cell Growth Factor 40

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.62-1000pg/mL

Sensitivity:

7.32pg/mL

Specificity:

This kit recognizes Human IL-9 in samples. No significant cross-reactivity or interference between Human IL-9 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| IL-9

Interleukin 9, also known as IL-9, is a pleiotropic cytokine (cell signalling molecule) belonging to the group of interleukins.IL-9 is produced by variety of cells like mast cells, NKT cells, Th2, Th17, Treg, ILC2, and Th9 cells in different amounts. Among them, Th9 cells are regarded as the major CD4+ T cells that produce IL-9.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.48	1.31	0.93	0.52	0.33	0.22	0.16	0.07
Corrected OD	2.41	1.24	0.86	0.45	0.26	0.15	0.09	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human IL-9 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	82-101	96
Plasma(n=8)	92-106	102
Cell Culture Supernates(n=8)	96-109	105

| Linearity

Samples were spiked with high concentrations of Human IL-9 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-95	88-96	90-110
1 : 2	Average (%)	91	93	98
1 : 4	Range (%)	89-103	87-108	103-115
1 : 4	Average (%)	94	96	108