| PDGF-C

Platelet-derived growth factor C, also known as PDGF-C, is a 345-amino acid protein thats is encoded by the PDGFC gene.Platelet-derived growth factors are important in connective tissue growth, survival and function, and consist of disulphide-linked dimers involving two polypeptide chains, PDGF-A and PDGF-B. PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique two-domain structure and expression pattern. PDGF-C was not previously identified with PDGF-A and PDGF-B, possibly because it may be that it is synthesized and secreted as a latent growth factor, requiring proteolytic removal of the N-terminal CUB domain for receptor binding and activation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	30	15	7.5	3.75	1.87	0.73	0.46	0
OD	2.25	1.64	1.08	0.71	0.46	0.33	0.25	0.08
Corrected OD	2.17	1.56	1	0.63	0.38	0.25	0.17	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human PDGF-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Plasma(n=8)	92-104	101

 

| Linearity

Samples were spiked with high concentrations of Human PDGF-C and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Plasma(n=4)
1 : 2	Range (%)	87-96
	Average (%)	92
1 : 4	Range (%)	87-108
	Average (%)	98