Human Sirtuin 6 (SIRT6) ELISA Kit

Cat.#:

JL15088

Regulatory Status:

RUO

Alternative:

SIR2L6; Silent Mating Type Information Regulation 2 Homolog 6; Mono-ADP-Ribosyltransferase Sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human SIRT6 in samples. No significant cross-reactivity or interference between Human SIRT6 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SIRT6

Sirtuin 6 (SIRT6 or Sirt6) is a stress responsive protein deacetylase and mono-ADP ribosyltransferase enzyme encoded by the SIRT6 gene. In laboratory research, SIRT6 appears to function in multiple molecular pathways related to aging, including DNA repair, telomere maintenance, glycolysis and inflammation. SIRT6 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.45	1.88	1.46	1.13	0.76	0.49	0.38	0.08
Corrected OD	2.37	1.8	1.38	1.05	0.68	0.41	0.3	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SIRT6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	96
Plasma(n=8)	93-105	102
Cell Culture Supernates(n=8)	96-108	103

| Linearity

Samples were spiked with high concentrations of Human SIRT6 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	85-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	100	108