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Human Sialyltransferase 10 (SIAT10) ELISA Kit

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Product Name:

Human Sialyltransferase 10 (SIAT10) ELISA Kit

Cat.#:

JL17584

Regulatory Status:
Alternative:

ST3GAL6; ST3GALVI; ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 6; Type 2 lactosamine alpha-2,3-sialyltransferase; beta-galactoside alpha-2,3-sialyltransferase VI

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.4ng/mL
Specificity:
This kit recognizes Human SIAT10 in samples. No significant cross-reactivity or interference between Human SIAT10 and analogues was observed.
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Cell Biology
  • Human Sialyltransferase 10 (SIAT10) ELISA Kit
  • Human Sialyltransferase 10 (SIAT10) ELISA Kit
  • Human Sialyltransferase 10 (SIAT10) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| SIAT10

Sialyltransferase 10 also called Alpha-2,3-Sialyltransferase VI. The protein encoded by this gene is a member of the sialyltransferase family. Members of this family are enzymes that transfer sialic acid from the activated cytidine 5'-monophospho-N-acetylneuraminic acid to terminal positions on sialylated glycolipids (gangliosides) or to the N- or O-linked sugar chains of glycoproteins. This protein has high specificity for neolactotetraosylceramide and neolactohexaosylceramide as glycolipid substrates and may contribute to the formation of selectin ligands and sialyl Lewis X, a carbohydrate important for cell-to-cell recognition and a blood group antigen.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 50 25 12.5 6.25 3.12 1.56 0.78 0
OD 2.08 1.58 1.06 0.7 0.43 0.3 0.23 0.08
Corrected OD 2 1.5 0.98 0.62 0.35 0.22 0.15 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human SIAT10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Tissue Homogenates(n=8) 83-101 96
Cell Culture Supernates(n=8) 92-105 103





 

| Linearity

Samples were spiked with high concentrations of Human SIAT10 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Tissue Homogenates(n=4) Cell Culture Supernates(n=4)
1 : 2 Range (%) 84-95 88-98
Average (%) 91 93
1 : 4 Range (%) 89-103 87-108
Average (%) 95 98







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
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