| TMLHe

Trimethyllysine dioxygenase, mitochondrial is an enzyme that is encoded by the TMLHE gene in chromosome X. Mutations in the TMLHE gene resulting in carnitine biosynthesis disruption have been associated with autism symptoms.The TMHLE gene is located at the extreme end of the Xq28 region with high genomic instability,[8] and encodes a protein trimethyllysine dioxygenase, a, Fe2+ and 2-oxoglytarate dependent non-heme-ferrous iron hydrolase localized to the mitochondrial matrix.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.6	1.48	0.94	0.57	0.39	0.26	0.18	0.07
Corrected OD	2.53	1.41	0.87	0.5	0.32	0.19	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human TMLHe spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-103	96
Cell Culture Supernates(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human TMLHe and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-98
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	95	98