| TGM1

Protein-glutamine gamma-glutamyltransferase K is a transglutaminase enzyme that is encoded by the TGM1 gene.Keratinocyte transglutaminase enzymes serve to specifically catalyze the development of the cornified cell envelope, a defining characteristic of epidermal keratinocytes that have undergone the termination of differentiation. The specific cross linkages formed by keratinocyte transglutaminase are between n^ε-(γ-glutamyl)lysine residues which develop into isopeptide protein-protein linkages that adds to the stabilization of the cornified cell envelope.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.44	1.87	1.29	0.83	0.58	0.38	0.27	0.08
Corrected OD	2.36	1.79	1.21	0.75	0.5	0.3	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human TGM1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human TGM1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	88-96
	Average (%)	93
1 : 4	Range (%)	87-107
	Average (%)	98