Human Neurotrophin 4 (NT-4) ELISA Kit

Cat.#:

JL11402

Regulatory Status:

RUO

Alternative:

NT5; NTF4; NT-4/5; Neurotrophic Factor 4; Neurotrophin-5

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

12.37pg/mL

Specificity:

This kit recognizes Human NT-4in samples. No significant cross-reactivity or interference between Human NT-4 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| NT-4

Neurotrophin-4 (NT-4),also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene.It is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase.NT-4 was first discovered and isolated from xenopus and viper in the year 1991 by Finn Hallbook.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.25	0
OD	2.29	1.64	1.08	0.7	0.44	0.28	0.19	0.08
Corrected OD	2.21	1.56	1	0.62	0.36	0.2	0.11	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human NT-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human NT-4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108