| CRYaB

Alpha-crystallin B chain is a protein that in humans is encoded by the CRYAB gene.It is part of the small heat shock protein family and functions as molecular chaperone that primarily binds misfolded proteins to prevent protein aggregation, as well as inhibit apoptosis and contribute to intracellular architecture.Post-translational modifications decrease the ability to chaperone.Mutations in CRYAB cause different cardiomyopathies,skeletal myopathiesmainly myofibrillar myopathy,and also cataracts.In addition, defects in this gene/protein have been associated with cancer and neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	40	20	10	5	2.5	1.25	0.62	0
OD	2.66	2.04	1.51	1.04	0.65	0.39	0.22	0.09
Corrected OD	2.57	1.95	1.42	0.95	0.56	0.3	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human CRYaB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human CRYaB and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98