| IGF-1R

Insulin Like Growth Factor 1 Receptor (IGF1R) is a transmembrane receptor that is activated by a hormone called insulin-like growth factor 1 (IGF-1) and by a related hormone called IGF-2. Two α and β subunits form the IGF-1 receptor, both of which are synthesized by an mRNA precursor. The precursor is then glycosylated, cleaved by proteolysis, and cross-linked by cysteine bonds to form a functional transmembrane αβ chain. During embryonic development, the IGF-1R pathway is involved with the developing limb buds.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.09	1.54	1.12	0.77	0.48	0.34	0.25	0.09
Corrected OD	2	1.45	1.03	0.68	0.39	0.25	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human IGF-1R spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-103	98
Cell Lysates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human IGF-1R and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	85-95	90-110
	Average (%)	91	96
1 : 4	Range (%)	89-105	105-115
	Average (%)	96	108