| PL-A1

Phospholipase A1 encoded by the PLA1A gene.PLA1's are present in numerous species including humans, and have a variety of cellular functions that include regulation and facilitation of the production of lysophospholipid mediators, and acting as digestive enzymes. These enzymes are responsible for fast turnover rates of cellular phospholipids.In addition to this, the products of the reaction catalyzed by PLA1 which are a fatty acid and a lysophospholipid are important in various biological functions such as platelet aggregation and smooth muscle contraction.In addition, lysophospholipids can be found as surfactants in food techniques and cosmetics, and can be used in drug delivery.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	2.29	1.64	1.08	0.7	0.44	0.28	0.19	0.08
Corrected OD	2.21	1.56	1	0.62	0.36	0.2	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human PL-A1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	93-105	102
Tissue Homogenates(n=8)	96-108	108

 

| Linearity

Samples were spiked with high concentrations of Human PL-A1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
	Average (%)	91	93	97
1 : 4	Range (%)	88-103	87-108	105-115
	Average (%)	93	98	108