| ADAMTS4

A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme encoded by the ADAMTS4 gene. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. It can degrade aggrecan, a major proteoglycan of cartilage, brevican, a brain-specific extracellular matrix protein, neurocan and versican. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	4000	2000	1000	500	250	125	62.5	0
OD	2.32	1.7	1.19	0.72	0.46	0.32	0.22	0.07
Corrected OD	2.25	1.63	1.12	0.65	0.39	0.25	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human ADAMTS4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	101
Tissue Homogenates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Human ADAMTS4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	95
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108