| Rat Anti-mouse PD-L1 Antibody

PD-L1, also known as CD274; B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, CD274 molecule, Programmed cell death ligand 1, hPD-L1, is a protein encoded by the CD274 gene.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.52	1.96	1.38	0.97	0.55	0.31	0.19	0.08
Corrected OD	2.44	1.88	1.3	0.89	0.47	0.23	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat Rat Anti-mouse PD-L1 Antibody spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	88-102	97
Plasma(n=8)	94-107	104

 

| Linearity

Samples were spiked with high concentrations of Rat Rat Anti-mouse PD-L1 Antibody and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	82-93	88-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-110
	Average (%)	94	98