| HP-IgG

Helicobacter pylori, previously known as Campylobacter pylori, is a gram-negative, flagellated, helical bacterium. Mutants can have a rod or curved rod shape that exhibits less virulence.The bacterium was first identified as the causal agent of gastric ulcers.Infection of the stomach with H. pylori does not necessarily cause illness: over half of the global population is infected, but most individuals are asymptomatic.Extragastric complications that have been linked to H. pylori include anemia due either to iron deficiency or vitamin B12 deficiency, diabetes mellitus, cardiovascular illness, and certain neurological disorders.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.52	1.96	1.38	0.96	0.55	0.31	0.19	0.08
Corrected OD	2.44	1.88	1.3	0.88	0.47	0.23	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat HP-IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	88-102	97
Plasma(n=8)	94-109	103

 

| Linearity

Samples were spiked with high concentrations of Rat HP-IgG and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	82-93	88-96
	Average (%)	92	92
1 : 4	Range (%)	89-104	87-110
	Average (%)	95	99