Product Name:
Pig Epidemic Diarrhoea Virus IgG (PEDV-IgG) ELISA Kit
Cat.#:
JL46524
Regulatory Status:
Alternative:
Epidemic Diarrhoea Virus IgG
Detection Method:
ELISA
Assay Type:
Indirect
Detection Range:
Qualitative analysis
Sensitivity:
Specificity:
This kit recognizes Pig PEDV-IgG in samples. No significant cross-reactivity or interference between Pig PEDV-IgG and analogues was observed.
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Assay Procedure:
Research Area:
Microbiology
Product Datasheets
| PEDV-IgG
Epidemic Diarrhea Virus (PEDV) is a single-stranded positive-sense RNA virus belonging to the Alphacoronavirus genus of the Coronaviridae family. The virion measures approximately 95-190 nm in diameter and features petal-shaped spike (S) protein projections on its surface. Its 28 kb genome encodes four structural proteins: S (spike), M (membrane), E (envelope), and N (nucleocapsid).PEDV primarily infects porcine small intestinal epithelial cells, causing watery diarrhea, vomiting, and dehydration. The mortality rate reaches 100% in suckling piglets, while growing and adult pigs exhibit milder symptoms with growth retardation. Transmission occurs mainly through fecal-oral routes, with indirect spread via fomites and vehicles. The virus demonstrates 1-7 days incubation period and can survive in feces for over 42 days.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Pig PEDV-IgG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Linearity
Samples were spiked with high concentrations of Pig PEDV-IgG and diluted with Universal Diluent to produce samples with values within the range of the assay.
Size
Quantity
Price ($)
48T
1
460 / 460
96T
1
760 / 760


