| AHA

Anti-histone antibodies are autoantibodies that are a subset of the anti-nuclear antibody family, which specifically target histone protein subunits or histone complexes.Anti-histone antibodies are used as a marker for systemic lupus erythematosus, but are also implicated in other autoimmune diseases like Sjögren syndrome, dermatomyositis, or rheumatoid arthritis.Anti-histone antibodies can be used as a marker for drug-induced lupus.Anti-histone antibodies are diverse, so aside from targeting the protein subunits, different antibodies may also be specific for different complexes, including the H2A-H2B dimer or the H3-H6 tetramer.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	200	100	50	25	12.5	6.25	3.12	0
OD	2.64	1.93	1.42	0.89	0.62	0.44	0.33	0.16
Corrected OD	2.48	1.77	1.26	0.73	0.46	0.28	0.17	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse AHA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	97
Plasma(n=8)	94-108	104

 

| Linearity

Samples were spiked with high concentrations of Mouse AHA and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	82-97	86-96
	Average (%)	92	93
1 : 4	Range (%)	89-103	87-111
	Average (%)	96	98