| dsDNA-Ab

Anti-double-stranded DNA antibody; Natural DNA antibody (dsDNA-Ab) is a group of antinuclear antibodies (ANA) whose target antigen is double-stranded DNA. They are highly diagnostic for systemic lupus erythematosus (SLE) and have been implicated in the pathogenesis of lupus nephritis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	25	12.5	6.25	3.12	1.56	0.78	0.39	0
OD	2.52	1.96	1.38	0.96	0.55	0.31	0.19	0.08
Corrected OD	2.44	1.88	1.3	0.88	0.47	0.23	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human dsDNA-Ab spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	96
Plasma(n=8)	92-105	100
Cell Culture Supernates(n=8)	96-109	105

 

| Linearity

Samples were spiked with high concentrations of Human dsDNA-Ab and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	92	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	93	99	108