| DHEA

Dehydroepiandrosterone (DHEA), also known as androstenolone, is an endogenous steroid hormone precursor.DHEA is produced in the adrenal glands,the gonads, and the brain.It functions as a metabolic intermediate in the biosynthesis of the androgen and estrogen sex steroids both in the gonads and in various other tissues.However, DHEA also has a variety of potential biological effects in its own right, binding to an array of nuclear and cell surface receptors,and acting as a neurosteroid and modulator of neurotrophic factor receptors.DHEA is produced in the zona reticularis of the adrenal cortex under the control of adrenocorticotropic hormone (ACTH) and by the gonads under the control of gonadotropin-releasing hormone (GnRH).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	0.188	0.27	0.389	0.642	0.929	1.38	1.766	1.842

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat DHEA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-102	97
Plasma(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Rat DHEA and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
	Average (%)	93	90
1 : 4	Range (%)	89-104	87-108
	Average (%)	96	98