| E

Estrone (E1) is a steroid, a weak estrogen, and a minor female sex hormone.It is one of three major endogenous estrogens, the others being estradiol and estriol.Estrone, as well as the other estrogens, are synthesized from cholesterol and secreted mainly from the gonads, though they can also be formed from adrenal androgens in adipose tissue.Relative to estradiol, both estrone and estriol have far weaker activity as estrogens.Estrone can be converted into estradiol, and serves mainly as a precursor or metabolic intermediate of estradiol.It is both a precursor and metabolite of estradiol.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.2	0
OD	0.216	0.298	0.417	0.669	0.955	1.405	1.79	1.865

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat E spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-102	97
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat E and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-97	90-110
	Average (%)	91	94	96
1 : 4	Range (%)	89-104	87-108	105-115
	Average (%)	93	98	110