| NTX

Type I Collagen Cross-Linked N-Telopeptide (NTX-I) is a stable end product of bone collagen degradation that appears in urine following hepatic processing. It primarily reflects osteoclast-mediated bone resorption activity and can sensitively detect changes in bone metabolism. As an important parameter for evaluating bone resorption in histomorphometric analysis, NTX-I is currently regarded as the most sensitive and specific marker for assessing bone resorption status. This biomarker is particularly valuable for monitoring conditions with altered bone turnover, such as osteoporosis and metastatic bone diseases, providing clinicians with a reliable tool for both diagnosis and treatment evaluation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	200	100	50	25	12.5	6.25	3.12	0
OD	0.216	0.298	0.417	0.669	0.955	1.405	1.79	1.865

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat NTX spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat NTX and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	93	98	110