| SSB

La/SSB antigen, also known as SSB/La, is a highly conserved phosphorylated protein within the cell nucleus. It is closely related to the previously mentioned SSB antigen but constitutes an independent entity in terms of molecular structure and function. Within the nucleus, SSB (Ro60) and SSB (La) are functionally interdependent. As a transcription termination factor, the La protein is responsible for receiving primary transcripts produced by RNA polymerase III, including Y RNAs bound to Ro60. Thus, SSB/Ro60-SSB/La forms a functionally interconnected unit in the RNA quality control pathway. This may explain why autoantibodies targeting them coexist so closely in serological studies.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	0.17	0.3	0.48	0.72	1.05	1.42	1.91	2.26

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse SSB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	88-105	96
Plasma(n=8)	94-106	101
Cell Culture Supernates(n=8)	96-110	105

 

| Linearity

Samples were spiked with high concentrations of Mouse SSB and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-94	88-96	90-110
	Average (%)	91	93	97
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	109