Mouse Fibrinopeptide A (FPA) ELISA Kit

Cat.#:

JL15777

Regulatory Status:

RUO

Alternative:

FP-A

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

1.56-100ng/mL

Sensitivity:

0.58ng/mL

Specificity:

This kit recognizes Mouse FPA in samples. No significant cross-reactivity or interference between Mouse FPA and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| FPA

Fibrinopeptide A is peptides which is located in the central region of the fibrous glycoprotein fibrinogen (factor I) and are cleaved by the enzyme thrombin (factor IIa) to convert fibrinogen into covalently-linked fibrin (factor IA) monomers.The N-terminal FpA is cleaved from the Aα chains of fibrinogen and FpB from the Bβ chains of fibrinogen, with FpA released before FpB.FpA is a 16-amino acid peptide.The half-life of FpA is very short at approximately 3 to 5 minutes.Hence, FpA levels provide a relatively transient measure of coagulation activation.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse FPA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Mouse FPA and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
1 : 4	Average (%)	93	98	110