| u-T3

Triiodothyronine, also known as T3, is a thyroid hormone.Production of T3 and its prohormone thyroxine (T4) is activated by thyroid-stimulating hormone (TSH), which is released from the anterior pituitary gland. At the cellular level, T3 is the body's more active and potent thyroid hormone.T3 helps deliver oxygen and energy to all of the body's cells, its effects on target tissues being roughly four times more potent than those of T4.Of the thyroid hormone that is produced, just about 20% is T3, whereas 80% is produced as T4.The half-life of T3 is about 2.5 days.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	0.17	0.28	0.49	0.91	1.31	1.72	1.95	2.37

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse u-T3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	82-101	97
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse u-T3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-104	86-108	105-115
	Average (%)	93	97	110