Human 4-Hydroxynonenal (4-HNE) ELISA Kit

Cat.#:

JL19014

Regulatory Status:

RUO

Alternative:

4-HNE

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.625-40ng/mL

Sensitivity:

0.319ng/mL

Specificity:

This kit recognizes Human 4-HNE in samples. No significant cross-reactivity or interference between Human 4-HNE and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| 4-HNE

4-Hydroxynonenal, or 4-hydroxy-2E-nonenal or 4-hydroxy-2-nonenal or 4-HNE or HNE, (C9H16O2), is an α,β-unsaturated hydroxyalkenal that is produced by lipid peroxidation in cells. 4-HNE is the primary α,β-unsaturated hydroxyalkenal formed in this process. It is a colorless oil. It is found throughout animal tissues, and in higher quantities during oxidative stress due to the increase in the lipid peroxidation chain reaction, due to the increase in stress events.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human 4-HNE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

| Linearity

Samples were spiked with high concentrations of Human 4-HNE and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
1 : 4	Average (%)	94	98