| 6-SMT

Melatonin, an indoleamine, is a natural compound produced by various organisms, including bacteria and eukaryotes.Melatonin is metabolized with an elimination half-life ranging from 20 to 50 minutes.The primary metabolic pathway transforms melatonin into 6-hydroxymelatonin, which is then conjugated with sulfate and excreted in urine as a waste product.6-hydroxymelatonin Sulfate (6-SMT) is the main excretion form of melatonin in urine.6-SMT as a stable terminal metabolite for the assessment of long-term melatonin secretion levels (e.g., in sleep disorders, depression diagnosis).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	250	125	62.5	31.2	15.6	7.8	3.9	0
OD	0.19	0.24	0.31	0.55	0.97	1.38	1.96	2.52

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human 6-SMT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-107	104

 

| Linearity

Samples were spiked with high concentrations of Human 6-SMT and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-105	87-108	105-115
	Average (%)	94	98	110