Human Reverse Triiodothyronine (rT3) ELISA Kit

Cat.#:

JL13137

Regulatory Status:

RUO

Alternative:

RT3; r-T3; Reverse T3;(2S)-2-Amino-3-[4-(4-Hydroxy-3;5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3;3';5'-Triiodo-L-thyronine

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

93.75-6000pg/mL

Sensitivity:

45.9pg/mL

Specificity:

This kit recognizes Human rT3 in samples. No significant cross-reactivity or interference between Human rT3 and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| rT3

Reverse triiodothyronine, also known as rT3, is an isomer of triiodothyronine (T3).Reverse T3 is the third-most common iodothyronine the thyroid gland releases into the bloodstream, at 0.9%; tetraiodothyronine (levothyroxine, T4) constitutes 90% and T3 is 9%. However, 95% of rT3 in human blood is made elsewhere in the body, as enzymes remove a particular iodine atom from T4.The levels of rT3 increase in conditions such as euthyroid sick syndrome because its clearance decreases while its production stays the same.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	6000	3000	1500	750	375	187.5	93.75	0
OD	0.188	0.27	0.389	0.642	0.929	1.38	1.766	1.842

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human rT3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

| Linearity

Samples were spiked with high concentrations of Human rT3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
1 : 4	Average (%)	93	98