Human Big Dynorphin (Dyn) ELISA Kit

Cat.#:

JL10192

Regulatory Status:

RUO

Alternative:

B-DYN; Biocytin 13 Dynorphin A 1-13 Amide

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

7.81-500pg/mL

Sensitivity:

3.7pg/mL

Specificity:

This kit recognizes Human Dyn in samples. No significant cross-reactivity or interference between Human Dyn and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| Dyn

Big dynorphin is an endogenous opioid peptide of the dynorphin family that is composed of both dynorphin A and dynorphin B.It has nociceptive and anxiolytic-like properties, as well as effects on memory in mice.Big dynorphin is a principal endogenous, agonist at the human kappa-opioid receptor.Dyn endogenous ligands for κ-opioid receptors. It has a wide distribution in the CNS including the hippocampus, amygdala, striatum, and spinal cord

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human Dyn spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Human Dyn and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-107	105-115
1 : 4	Average (%)	93	98	110