Human Free Thyroxine (FT4) ELISA Kit

Cat.#:

JL20023

Regulatory Status:

RUO

Alternative:

FT4

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

1-64pmol/L

Sensitivity:

0.7pmol/L

Specificity:

This kit recognizes Human FT4 in samples. No significant cross-reactivity or interference between Human FT4 and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| FT4

Thyroxine, also known as T4, is a hormone produced by the thyroid gland. It is the primary form of thyroid hormone found in the blood and acts as a prohormone of the more active thyroid hormone, triiodothyronine (T3).The half-life of T4 is about 6.5 days.Thyroxine biosynthesis is a multi-step process that occurs in follicular cell within the thyroid gland. The synthesis of thyroxine requires adequate iodine supply and appropriate hormonal control.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human FT4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

| Linearity

Samples were spiked with high concentrations of Human FT4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
1 : 4	Average (%)	93	98